MIcro-Nanofluidics for Life science and Environment

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MILE team addresses biophysics-related scientific questions thanks to microfluidic technologies. We focus on the interplay between permeability, mechanical properties, and biological activity of tissues and cells. Applications range from the role of mechanics in cancer to filtration, and cell sorting in the blood.

We address biophysics-related scientific questions thanks to microfluidic technologies. See our Research topics below.

Microvasculature on chip: deformation, permeation and immunomodulation of lymphatic and blood vessels

We study the barrier and immunomodulation functions of vascular tissues with organ-on-chip technologies.


Mechanics of multicellular aggregates, and their interactions with nanoparticles

We develop an original fluidic technology for measuring the mechanical properties of cell aggregates (micropipettes on a chip), fundamental to many biological processes.


Cells and extracellular vesicles: Microfluidics for their purification and the analysis of their nucleic acid composition

We develop microfluidic technologies for purifying cells and extracellular vesicles.


Impact of spatial confinement on cell physiology

We use microfluidics to study the impact of confinement on the behavior of living cells.


(Bio)filtration-on-chip, or how to study microscale filtration mechanisms.

We use microfluidic models of filtration membranes to study bio-clogging.


Latest publications


Journal articles

Terence Desclaux, Leonardo Santana, Inès Verdeille, Paul Duru, Pierre Joseph, et al.. Hydrodynamic resistance of a yeast clog. Physical Review Fluids, 2024, 9 (3), pp.034202. ⟨10.1103/PhysRevFluids.9.034202⟩. ⟨hal-04224639v4⟩

Sylvain Landiech, Marianne Elias, Pierre Lapèze, Hajar Ajiyel, Marine Plancke, et al.. Parallel on-chip micropipettes enabling quantitative multiplexed characterization of vesicle mechanics and cell aggregates rheology. APL Bioengineering, 2024, 8 (2), pp.026122. ⟨10.1063/5.0193333⟩. ⟨hal-04616249⟩


Journal articles

Jean Cacheux, Jose Ordonez-Miranda, Aurélien Bancaud, Laurent Jalabert, Daniel Alcaide, et al.. Asymmetry of tensile versus compressive elasticity and permeability contributes to the regulation of exchanges in collagen gels. Science Advances , 2023, 9 (31), ⟨10.1126/sciadv.adf9775⟩. ⟨hal-04235287⟩

Victor Fournié, Bastien Venzac, Emmanuelle Trevisiol, Julie Foncy, Julien Roul, et al.. A microfluidics-assisted photopolymerization method for high-resolution multimaterial 3D printing. Additive Manufacturing, 2023, 72, pp.103629. ⟨10.1016/j.addma.2023.103629⟩. ⟨hal-04116522⟩

Daniel Alcaide, Jean Cacheux, Aurélien Bancaud, Rieko Muramatsu, Yukiko T Matsunaga. Solute transport in the brain tissue: what are the key biophysical parameters tying in vivo and in vitro studies together?. Biomaterials Science, 2023, 10, pp.3450-3460. ⟨10.1039/D3BM00027C⟩. ⟨hal-04063141⟩

Liam Holt, Morgan Delarue. Macromolecular crowding: Sensing without a sensor. Current Opinion in Cell Biology, 2023, 85, pp.102269. ⟨10.1016/j.ceb.2023.102269⟩. ⟨hal-04270738⟩

Zacchari Ben Meriem, Tiphaine Mateo, Julien Faccini, Céline Denais, Romane Dusfour-Castan, et al.. A microfluidic mechano-chemostat for tissues and organisms reveals that confined growth is accompanied with increased macromolecular crowding. Lab on a Chip, 2023, 20, pp.4445-4455. ⟨10.1039/d3lc00313b⟩. ⟨hal-04214597⟩

Jean Cacheux, Aurélien Bancaud, Daniel Alcaide, Jun-Ichi Suehiro, Yoshihiro Akimoto, et al.. Endothelial tissue remodeling induced by intraluminal pressure enhances paracellular solute transport. iScience, 2023, 26 (7), pp.107141. ⟨10.1016/j.isci.2023.107141⟩. ⟨hal-04250976⟩

Paul Bruand, Inga Tijunelyte, Adrien Castinel, Cécile Donnadieu, Pierre Joseph, et al.. Size Fractionation of Milliliter DNA Samples in Minutes Controlled by an Electric Field of ∼10 V. Analytical Chemistry, 2023, 95 (49), pp.18099-18106. ⟨10.1021/acs.analchem.3c03187⟩. ⟨hal-04322873⟩

Conference papers

Tiphaine Mateo, Morgane Delarue, Fabien Mesnilgrente, Pierre-François Calmon. Lithographie laser bi-photons pour l'impression de moules PDMS dédiés à des applications micro-fluidique. Renatech Litho Gravure, Réseau Renatech, Jan 2023, Lille, France. ⟨hal-04006407⟩

Other documents

Mohammad Hussein Baz, Lucas Charpin, Coralie Sengenès, Emmanuelle Trévisiol, Anne Marie Gué. Lab-on-a-chip for Isolating Circulating Adipose Stromal Cells: Pre-processing Module. International Symposium on Microscale Separations and Bioanalysis (MSB), May 2023, Tallahassee, Florida, United States. , 2023. ⟨hal-04117613⟩


Journal articles

Vincent Gerbaud, Hugues Leiser, Johnny Beaugrand, Bernard Cathala, Carole Molina-Jouve, et al.. Bibliometric survey and network analysis of biomimetics and nature inspiration in engineering science. Bioinspiration and Biomimetics, 2022, pp.ac4f2e. ⟨10.1088/1748-3190/ac4f2e⟩. ⟨hal-03561416⟩

Inga Tijunelyte, Jeffrey Teillet, Paul Bruand, Rémi Courson, Aurélie Lecestre, et al.. Hybridization-based DNA biosensing with a limit of detection of 4 fM in 30 s using an electrohydrodynamic concentration module fabricated by grayscale lithography. Biomicrofluidics, 2022, 16 (4), pp.044111. ⟨10.1063/5.0073542⟩. ⟨hal-03771547⟩

Baptiste Alric, Cécile Formosa-Dague, Etienne Dague, Liam J Holt, Morgan Delarue. Macromolecular crowding limits growth under pressure. Nature Physics, 2022, ⟨10.1038/s41567-022-01506-1⟩. ⟨hal-03428857⟩

Conference papers

Paul Bruand, Inga Tijunelyte, Frédéric Ginot, Pierre Joseph, Aurélien Bancaud. Purification and size-fractionation of milli-liter DNA samples in minutes using electrohydrodynamic migration. MSB 2022 (Internation Synmposium on Miscroscale Separations and Bioanalysis), Jul 2022, Liège, Belgium. ⟨hal-03818343⟩

Jean Cacheux, Aurélien Bancaud, Jose Ordonez-Miranda, Laurent Jalabert, Yukiko T Matsunaga. Collagen matrix remodeling by fibroblasts monitored by interstitial flow metrology. Microphysiological systems: from organoids to organ-on-chip, Apr 2022, Cargèse, France. ⟨hal-03821244⟩

Aurélien Bancaud. Deformation but not convection induced by intraluminal pressure enhances transport through barrier tissues. BTI : first international conference on Blood Tissue Interfaces, Oct 2022, Montpellier, France. ⟨hal-03821260⟩

Other documents

Mohammad Hussein Baz, Lucas Charpin, Audrey Varin, Coralie Sengenès, Emmanuelle Trevisiol, et al.. Lab-on-a-Chip for the isolation of circulating Adipose Stem Cells. GDR Micro Nanofluidics GDR Bio ingénierie des Interfaces, Oct 2022, Paris, France. 2022. ⟨hal-03823431⟩

Preprints, Working Papers, ...

M. Di-Luoffo, C. Schmitter, E.C. Barrere, N. Therville, Morgan Delarue, et al.. The biomechanical context influences the PI3K output signaling in breast and pancreatic cancer cells. 2022. ⟨hal-03808261⟩

Tong Shu, Tamás Szórádi, Gururaj Kidiyoor, Ying Xie, Nora Herzog, et al.. nucGEMs probe the biophysical properties of the nucleoplasm. 2022. ⟨hal-03808263⟩


Journal articles

Delphine Bordignon, Barbara Lonetti, Christophe Coudret, Pierre Roblin, Pierre Joseph, et al.. Wet spinning of a library of carbohydrate low molecular weight gels. Journal of Colloid and Interface Science, 2021, 603, pp.333 - 343. ⟨10.1016/j.jcis.2021.06.058⟩. ⟨hal-03278411⟩

Inga Tijunelyte, Rémi Malbec, Bayan Chami, Jean Cacheux, Christophe Dez, et al.. micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation. Biosensors and Bioelectronics, 2021, 178, pp.112992. ⟨10.1016/j.bios.2021.112992⟩. ⟨hal-03449104⟩

Laurent Malaquin, Morgan Delarue, Anne Marie Gué. Les « systèmes micro-physiologique » : des outils pour modéliser le vivant. Le petit illustré, regards croisés de chercheur.es, 2021. ⟨hal-03459155⟩

Venkata Suresh Vajrala, Baptiste Alric, Adrian Laborde, Camille Colin, Emmanuel Suraniti, et al.. Microwell Array Based Opto‐Electrochemical Detections Revealing Co‐Adaptation of Rheological Properties and Oxygen Metabolism in Budding Yeast. Advanced Biology, 2021, 5 (7), pp.2100484. ⟨10.1002/adbi.202100484⟩. ⟨hal-03228340⟩

M. Di-Luoffo, Zacchari Ben-Meriem, P. Lefebvre, Morgan Delarue, Julie Guillermet-Guibert. PI3K functions as a hub in mechanotransduction. Trends in Biochemical Sciences, 2021, 46 (11), pp.878-888. ⟨10.1016/j.tibs.2021.05.005⟩. ⟨hal-03252135⟩

Conference papers

Lyne Pillemont, Daniel Guneysu, Wilfrid Boireau, Céline Elie-Caille, Anne Marie Gué. A LOW-COST AND EASY TO USE SORTING DEVICE FOR THE SEPARATION OF EXTRACELLULAR VESICLES FROM COMPLEX FLUIDS. MicroTAS 2021, Amy E. Herr, Joel Voldman, Oct 2021, Palm Springs, United States. ⟨hal-03514431⟩

Other documents

Lyne Pillemont, Daniel Guneysu, Wilfrid Boireau, Celine Elie-Caille, Anne Marie Gué. A LOW-COST AND EASY TO USE SORTING DEVICE FOR THE SEPARATION OF EXTRACELLULAR VESICLES FROM COMPLEX FLUIDS. MicroTAS 2021, Oct 2021, Palm Spring, United States. , MicroTAS 2021, 2021, The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. ⟨hal-03514467⟩

Térence Desclaux, Paul Duru, Pierre Joseph, Morgan Delarue, Olivier Liot. CLOGGING OF A MODEL PORE BY A YEAST SUSPENSION (SACCHAROMYCES CEREVISIAE). Scientific meeting of the Micro-Nano-Fluidics Network GDR MNF, Sep 2021, Toulouse, France. ⟨hal-03384866⟩


Journal articles

Anaïs Chalard, Morgane Mauduit, Sandrine Assié-Souleille, Pierre Joseph, Laurent Malaquin, et al.. 3D printing of a biocompatible low molecular weight supramolecular hydrogel by dimethylsulfoxide water solvent exchange. Additive Manufacturing, 2020, 33, pp.101162. ⟨10.1016/j.addma.2020.101162⟩. ⟨hal-02540295⟩

Ilaria Francesca Rizzuti, Pietro Mascheroni, Silvia Arcucci, Zacchari Ben-Mériem, Audrey Prunet, et al.. Mechanical Control of Cell Proliferation Increases Resistance to Chemotherapeutic Agents. Physical Review Letters, 2020, 125 (12), pp.128103. ⟨10.1103/PhysRevLett.125.128103⟩. ⟨hal-02943906⟩

Bayan Chami, Nicolas Milon, Juan-Luis Fuentes Rojas, Samuel Charlot, Jean-Christophe Marrot, et al.. Single-step electrohydrodynamic separation of 1–150 kbp in less than 5 min using homogeneous glass/adhesive/glass microchips. Talanta, 2020, 217, pp.121013. ⟨10.1016/j.talanta.2020.121013⟩. ⟨hal-03024740⟩

Marianne Elias, Adrien Dutoya, Adrian Laborde, Aurélie Lecestre, Costanza Montis, et al.. Microfluidic characterization of biomimetic membrane mechanics with an on-chip micropipette. Micro and Nano Engineering, 2020, 8, pp.100064. ⟨10.1016/j.mne.2020.100064⟩. ⟨hal-02937882⟩

Costanza Montis, Pierre Joseph, Chiara Magnani, Alejandro Marín-Menéndez, Francesco Barbero, et al.. Multifunctional nanoassemblies target bacterial lipopolysaccharides for enhanced antimicrobial DNA delivery. Colloids and Surfaces B: Biointerfaces, 2020, 195, pp.111266. ⟨10.1016/j.colsurfb.2020.111266⟩. ⟨hal-02949343⟩

Jeffrey Teillet, Quentin Martinez, Inga Tijunelyte, Bayan Chami, Aurélien Bancaud. Characterization and minimization of band broadening in DNA electrohydrodynamic migration for enhanced size separation. Soft Matter, 2020, 16 (24), pp.5640-5649. ⟨10.1039/D0SM00475H⟩. ⟨hal-03024741⟩

Mohand Larbi Mokrane, Térence Desclaux, Jeffrey F. Morris, Pierre Joseph, Olivier Liot. Microstructure of the near-wall layer of filtration-induced colloidal assembly. Soft Matter, 2020, 42, ⟨10.1039/D0SM01143F⟩. ⟨hal-02879712⟩

Nicolas Milon, Juan-Luis Fuentes Rojas, Adrien Castinel, Laurent Bigot, Géraud Bouwmans, et al.. A tunable filter for high molecular weight DNA selection and linked-read sequencing. Lab on a Chip, 2020, 20 (1), pp.175-184. ⟨10.1039/C9LC00965E⟩. ⟨hal-02400601⟩

Preprints, Working Papers, ...

Olivier Liot, Catherine Sempere, Christophe Ybert, Anne-Laure Biance. Coupled electrokinetic transport through a nanoporous membrane: a theoretical approach. 2020. ⟨hal-02900453⟩


Journal articles

Nicolas Milon, Céline Chantry-Darmon, Carine Satge, Margaux-Alison Fustier, Stéphane Cauet, et al.. μLAS technology for DNA isolation coupled to Cas9-assisted targeting for sequencing and assembly of a 30 kb region in plant genome. Nucleic Acids Research, 2019, 47 (15), pp.8050-8060. ⟨10.1093/nar/gkz632⟩. ⟨hal-02338524⟩

Marius Socol, Hubert Ranchon, Bayan Chami, Antony Lesage, Jean-Marc Victor, et al.. Contraction and Tumbling Dynamics of DNA in Shear Flows under Confinement Induced by Transverse Viscoelastic Forces. Macromolecules, 2019, 52 (4), pp.1843-1852. ⟨10.1021/acs.macromol.8b02184⟩. ⟨hal-02076283⟩

Antoine Naillon, Pierre Joseph, Marc Prat. Direct observation of pore collapse and tensile stress generation on pore walls due to salt crystallization in a PDMS channel. Soft Matter, 2019, 15, pp.4562-4569. ⟨10.1039/C8SM02546K⟩. ⟨hal-02363335⟩

Marius Socol, Renjie Wang, Daniel Jost, Pascal Carrivain, Cédric Vaillant, et al.. Rouse model with transient intramolecular contacts on a timescale of seconds recapitulates folding and fluctuation of yeast chromosomes. Nucleic Acids Research, 2019, 47 (12), pp.6195-6207. ⟨10.1093/nar/gkz374⟩. ⟨hal-02276944⟩

Rémi Malbec, Bayan Chami, Lorène Aeschbach, Gustavo Ruiz Buendía, Marius Socol, et al.. µLAS: Sizing of expanded trinucleotide repeats with femtomolar sensitivity in less than 5 minutes. Scientific Reports, 2019, 9, pp.23. ⟨10.1038/s41598-018-36632-5⟩. ⟨hal-01997319⟩

Anaïs Chalard, Pierre Joseph, Sandrine Assié-Souleille, Barbara Lonetti, Nathalie Saffon-Merceron, et al.. Wet spinning and radial self-assembly of a carbohydrate low molecular weight gelator into well organized hydrogel filaments. Nanoscale, 2019, ⟨10.1039/C9NR02727K⟩. ⟨hal-02166311⟩

Jean Cacheux, Aurélien Bancaud, Thierry Leichle, Pierre Cordelier. Technological Challenges and Future Issues for the Detection of Circulating MicroRNAs in Patients With Cancer. Frontiers in Chemistry, 2019, 7, ⟨10.3389/fchem.2019.00815⟩. ⟨hal-02392369⟩

Loic Recoules, Sulivan Jouanneau, Gerald Thouand, Anne Marie Gué, Ali Boukabache. Towards a Miniaturized Device to Evaluate the BOD Parameter of Wastwater. International Journal of Environmental Science and Development, 2019, 10 (6), pp.178-182. ⟨10.18178/ijesd.2019.10.6.1168⟩. ⟨hal-02353122⟩

Conference papers

Lyne Pillemont, Daniel Guneysu, Céline Elie-Caille, Wilfrid Boireau, Anne Marie Gué. Towards on-chip EVs separation: a lab-on-chip approach. Annual meeting of the International Society for Extracellular Vesicles (ISEV2019), International Society for Extracellular Vesicles, Apr 2019, Kyoto, Japan. pp.PS04.10. ⟨hal-02421493⟩

Pierre Joseph, Marianne Elias, Fabien Mesnilgrente, Adrian Laborde, Debora Berti, et al.. Microfluidics to fabricate and probe vesicles mimicking cells. BioPhee - Membrane Biophysics of Exo-Endocytosis: From Model Systems to Cells, Apr 2019, Mandelieu la Napoule, France. ⟨hal-02166276⟩

Marion Valette, Mathias Bouguelmouna, Mélanie Mariotte, Rémi Courson, Marie-Charline Blatché, et al.. ON-CHIP PRE-TREATMENT OF BIOLOGICAL SAMPLES FOR THE ISOLATION OF ADIPOSE STEM CELLS (ASCs). Transducers, Jun 2019, Berlin, Germany. ⟨10.1109/TRANSDUCERS.2019.8808694⟩. ⟨hal-02190131⟩

Lyne Pillemont, Daniel Guneysu, Céline Elie-Caille, Wilfrid Boireau, Anne Marie Gué. Towards onchip EVs separation: a lab‐on‐chip approach. J Extracell Vesicles. International Society for Extracellular Vesicles, Apr 2019, Kyoto, Japan. ⟨hal-02991632⟩

Other documents

Marion Valette, Mathias Bouguelmouna, Mélanie Mariotte, Rémi Courson, Marie-Charline Blatché, et al.. Adipose Stem Cells (ASCs) isolation by on-chip pre-treatment of biological samples. Lab-On-Chip and Microfluidics Europe, Jun 2019, Rotterdam, Netherlands. 2019. ⟨hal-02190393⟩

We collaborate with several groups working on oncology and biophysics. We lead the ERC Project UnderPressure, and participate to several European and National ANR projects. See Research projects pages for details.

We are also strongly involved in the French National Network on Microfluidics GDR Micro Nano Fluidics MNF



Notre équipe de recherche

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