Micro-Nanofluidique pour les sciences de la vie et de l’environnement

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L’équipe MILE aborde des questions de biophysique grâce aux technologies microfluidiques. Nous étudions le lien entre perméabilité, propriétés mécaniques, et activité biologique de tissus et cellules. Les applications vont du rôle de la mécanique dans les cancers, à la filtration et au tri cellulaire.


L’équipe aborde des questions de biophysique grâce aux technologies microfluidiques. Nos sujets de recherche sont explicités ci-dessous.

Impact du confinement spatial sur la physiologie cellulaire

Nous utilisons la microfluidique pour étudier l'impact du confinement sur le comportement de cellules vivantes.

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Mécanique d'agrégats multicellulaires, et leurs interactions avec des nanoparticules

Nous développons une technologie fluidique originale de mesure des propriétés mécaniques d’agrégats cellulaires (micropipettes sur puce), fondamentales pour de nombreux processus biologiques.

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Cellules et vésicules extracellulaires : Microfluidique pour leur purification et l'analyse de leur composition en acides nucléiques

Nous développons des technologies microfluidiques pour purifier des cellules et des vésicules extracellulaires.

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Microvasculature sur puce : déformation, perméation et immunomodulation des vaisseaux lymphatiques et sanguins

Nous étudions les fonctions de barrière et d'immunomodulation de ces tissus vasculaires à l'aide de technologies d'organes sur puce.

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(Bio)filtration sur puce, ou comment étudier les mécanismes de filtration à micro-échelle.

Nous étudions le biocolmatage grâce à des modèles microfluidiques de membranes de filtration

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Dernières Publications

2024

Articles dans une revue

Terence Desclaux, Leonardo Santana, Inès Verdeille, Paul Duru, Pierre Joseph, et al.. Hydrodynamic resistance of a yeast clog. Physical Review Fluids, 2024, 9 (3), pp.034202. ⟨10.1103/PhysRevFluids.9.034202⟩. ⟨hal-04224639v4⟩

2023

Articles dans une revue

Paul Bruand, Inga Tijunelyte, Adrien Castinel, Cécile Donnadieu, Pierre Joseph, et al.. Size Fractionation of Milliliter DNA Samples in Minutes Controlled by an Electric Field of ∼10 V. Analytical Chemistry, 2023, 95 (49), pp.18099-18106. ⟨10.1021/acs.analchem.3c03187⟩. ⟨hal-04322873⟩

Jean Cacheux, Jose Ordonez-Miranda, Aurélien Bancaud, Laurent Jalabert, Daniel Alcaide, et al.. Asymmetry of tensile versus compressive elasticity and permeability contributes to the regulation of exchanges in collagen gels. Science Advances , 2023, 9 (31), ⟨10.1126/sciadv.adf9775⟩. ⟨hal-04235287⟩

Victor Fournié, Bastien Venzac, Emmanuelle Trevisiol, Julie Foncy, Julien Roul, et al.. A microfluidics-assisted photopolymerization method for high-resolution multimaterial 3D printing. Additive Manufacturing, 2023, 72, pp.103629. ⟨10.1016/j.addma.2023.103629⟩. ⟨hal-04116522⟩

Liam Holt, Morgan Delarue. Macromolecular crowding: Sensing without a sensor. Current Opinion in Cell Biology, 2023, 85, pp.102269. ⟨10.1016/j.ceb.2023.102269⟩. ⟨hal-04270738⟩

Zacchari Ben Meriem, Tiphaine Mateo, Julien Faccini, Céline Denais, Romane Dusfour-Castan, et al.. A microfluidic mechano-chemostat for tissues and organisms reveals that confined growth is accompanied with increased macromolecular crowding. Lab on a Chip, 2023, 20, pp.4445-4455. ⟨10.1039/d3lc00313b⟩. ⟨hal-04214597⟩

Jean Cacheux, Aurélien Bancaud, Daniel Alcaide, Jun-Ichi Suehiro, Yoshihiro Akimoto, et al.. Endothelial tissue remodeling induced by intraluminal pressure enhances paracellular solute transport. iScience, 2023, 26 (7), pp.107141. ⟨10.1016/j.isci.2023.107141⟩. ⟨hal-04250976⟩

Daniel Alcaide, Jean Cacheux, Aurélien Bancaud, Rieko Muramatsu, Yukiko T Matsunaga. Solute transport in the brain tissue: what are the key biophysical parameters tying in vivo and in vitro studies together?. Biomaterials Science, 2023, 10, pp.3450-3460. ⟨10.1039/D3BM00027C⟩. ⟨hal-04063141⟩

Communications dans un congrès

Tiphaine Mateo, Morgane Delarue, Fabien Mesnilgrente, Pierre-François Calmon. Lithographie laser bi-photons pour l'impression de moules PDMS dédiés à des applications micro-fluidique. Renatech Litho Gravure, Réseau Renatech, Jan 2023, Lille, France. ⟨hal-04006407⟩

Autres documents

Mohammad Hussein Baz, Lucas Charpin, Coralie Sengenès, Emmanuelle Trévisiol, Anne Marie Gué. Lab-on-a-chip for Isolating Circulating Adipose Stromal Cells: Pre-processing Module. International Symposium on Microscale Separations and Bioanalysis (MSB), May 2023, Tallahassee, Florida, United States. , 2023. ⟨hal-04117613⟩

2022

Articles dans une revue

Vincent Gerbaud, Hugues Leiser, Johnny Beaugrand, Bernard Cathala, Carole Molina-Jouve, et al.. Bibliometric survey and network analysis of biomimetics and nature inspiration in engineering science. Bioinspiration and Biomimetics, 2022, pp.ac4f2e. ⟨10.1088/1748-3190/ac4f2e⟩. ⟨hal-03561416⟩

Baptiste Alric, Cécile Formosa-Dague, Etienne Dague, Liam J Holt, Morgan Delarue. Macromolecular crowding limits growth under pressure. Nature Physics, 2022, ⟨10.1038/s41567-022-01506-1⟩. ⟨hal-03428857⟩

Inga Tijunelyte, Jeffrey Teillet, Paul Bruand, Rémi Courson, Aurélie Lecestre, et al.. Hybridization-based DNA biosensing with a limit of detection of 4 fM in 30 s using an electrohydrodynamic concentration module fabricated by grayscale lithography. Biomicrofluidics, 2022, 16 (4), pp.044111. ⟨10.1063/5.0073542⟩. ⟨hal-03771547⟩

Communications dans un congrès

Paul Bruand, Inga Tijunelyte, Frédéric Ginot, Pierre Joseph, Aurélien Bancaud. Purification and size-fractionation of milli-liter DNA samples in minutes using electrohydrodynamic migration. MSB 2022 (Internation Synmposium on Miscroscale Separations and Bioanalysis), Jul 2022, Liège, Belgium. ⟨hal-03818343⟩

Aurélien Bancaud. Deformation but not convection induced by intraluminal pressure enhances transport through barrier tissues. BTI : first international conference on Blood Tissue Interfaces, Oct 2022, Montpellier, France. ⟨hal-03821260⟩

Autres documents

Mohammad Hussein Baz, Lucas Charpin, Audrey Varin, Coralie Sengenès, Emmanuelle Trevisiol, et al.. Lab-on-a-Chip for the isolation of circulating Adipose Stem Cells. GDR Micro Nanofluidics GDR Bio ingénierie des Interfaces, Oct 2022, Paris, France. 2022. ⟨hal-03823431⟩

Pré-publications, documents de travail

Tong Shu, Tamás Szórádi, Gururaj Kidiyoor, Ying Xie, Nora Herzog, et al.. nucGEMs probe the biophysical properties of the nucleoplasm. 2022. ⟨hal-03808263⟩

M. Di-Luoffo, C. Schmitter, E.C. Barrere, N. Therville, Morgan Delarue, et al.. The biomechanical context influences the PI3K output signaling in breast and pancreatic cancer cells. 2022. ⟨hal-03808261⟩

2021

Articles dans une revue

Venkata Suresh Vajrala, Baptiste Alric, Adrian Laborde, Camille Colin, Emmanuel Suraniti, et al.. Microwell Array Based Opto‐Electrochemical Detections Revealing Co‐Adaptation of Rheological Properties and Oxygen Metabolism in Budding Yeast. Advanced Biology, 2021, 5 (7), pp.2100484. ⟨10.1002/adbi.202100484⟩. ⟨hal-03228340⟩

M. Di-Luoffo, Zacchari Ben-Meriem, P. Lefebvre, Morgan Delarue, Julie Guillermet-Guibert. PI3K functions as a hub in mechanotransduction. Trends in Biochemical Sciences, 2021, 46 (11), pp.878-888. ⟨10.1016/j.tibs.2021.05.005⟩. ⟨hal-03252135⟩

Inga Tijunelyte, Rémi Malbec, Bayan Chami, Jean Cacheux, Christophe Dez, et al.. micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation. Biosensors and Bioelectronics, 2021, 178, pp.112992. ⟨10.1016/j.bios.2021.112992⟩. ⟨hal-03449104⟩

Delphine Bordignon, Barbara Lonetti, Christophe Coudret, Pierre Roblin, Pierre Joseph, et al.. Wet spinning of a library of carbohydrate low molecular weight gels. Journal of Colloid and Interface Science, 2021, 603, pp.333 - 343. ⟨10.1016/j.jcis.2021.06.058⟩. ⟨hal-03278411⟩

Laurent Malaquin, Morgan Delarue, Anne Marie Gué. Les « systèmes micro-physiologique » : des outils pour modéliser le vivant. Le petit illustré, regards croisés de chercheur.es, 2021. ⟨hal-03459155⟩

Communications dans un congrès

Lyne Pillemont, Daniel Guneysu, Wilfrid Boireau, Céline Elie-Caille, Anne Marie Gué. A LOW-COST AND EASY TO USE SORTING DEVICE FOR THE SEPARATION OF EXTRACELLULAR VESICLES FROM COMPLEX FLUIDS. MicroTAS 2021, Amy E. Herr, Joel Voldman, Oct 2021, Palm Springs, United States. ⟨hal-03514431⟩

Autres documents

Lyne Pillemont, Daniel Guneysu, Wilfrid Boireau, Celine Elie-Caille, Anne Marie Gué. A LOW-COST AND EASY TO USE SORTING DEVICE FOR THE SEPARATION OF EXTRACELLULAR VESICLES FROM COMPLEX FLUIDS. MicroTAS 2021, Oct 2021, Palm Spring, United States. , MicroTAS 2021, 2021, The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. ⟨hal-03514467⟩

Térence Desclaux, Paul Duru, Pierre Joseph, Morgan Delarue, Olivier Liot. CLOGGING OF A MODEL PORE BY A YEAST SUSPENSION (SACCHAROMYCES CEREVISIAE). Scientific meeting of the Micro-Nano-Fluidics Network GDR MNF, Sep 2021, Toulouse, France. ⟨hal-03384866⟩

2020

Articles dans une revue

Mohand Larbi Mokrane, Térence Desclaux, Jeffrey F. Morris, Pierre Joseph, Olivier Liot. Microstructure of the near-wall layer of filtration-induced colloidal assembly. Soft Matter, 2020, 42, ⟨10.1039/D0SM01143F⟩. ⟨hal-02879712⟩

Ilaria Francesca Rizzuti, Pietro Mascheroni, Silvia Arcucci, Zacchari Ben-Mériem, Audrey Prunet, et al.. Mechanical Control of Cell Proliferation Increases Resistance to Chemotherapeutic Agents. Physical Review Letters, 2020, 125 (12), pp.128103. ⟨10.1103/PhysRevLett.125.128103⟩. ⟨hal-02943906⟩

Bayan Chami, Nicolas Milon, Juan-Luis Fuentes Rojas, Samuel Charlot, Jean-Christophe Marrot, et al.. Single-step electrohydrodynamic separation of 1–150 kbp in less than 5 min using homogeneous glass/adhesive/glass microchips. Talanta, 2020, 217, pp.121013. ⟨10.1016/j.talanta.2020.121013⟩. ⟨hal-03024740⟩

Marianne Elias, Adrien Dutoya, Adrian Laborde, Aurélie Lecestre, Costanza Montis, et al.. Microfluidic characterization of biomimetic membrane mechanics with an on-chip micropipette. Micro and Nano Engineering, 2020, 8, pp.100064. ⟨10.1016/j.mne.2020.100064⟩. ⟨hal-02937882⟩

Costanza Montis, Pierre Joseph, Chiara Magnani, Alejandro Marín-Menéndez, Francesco Barbero, et al.. Multifunctional nanoassemblies target bacterial lipopolysaccharides for enhanced antimicrobial DNA delivery. Colloids and Surfaces B: Biointerfaces, 2020, 195, pp.111266. ⟨10.1016/j.colsurfb.2020.111266⟩. ⟨hal-02949343⟩

Jeffrey Teillet, Quentin Martinez, Inga Tijunelyte, Bayan Chami, Aurélien Bancaud. Characterization and minimization of band broadening in DNA electrohydrodynamic migration for enhanced size separation. Soft Matter, 2020, 16 (24), pp.5640-5649. ⟨10.1039/D0SM00475H⟩. ⟨hal-03024741⟩

Anaïs Chalard, Morgane Mauduit, Sandrine Assié-Souleille, Pierre Joseph, Laurent Malaquin, et al.. 3D printing of a biocompatible low molecular weight supramolecular hydrogel by dimethylsulfoxide water solvent exchange. Additive Manufacturing, 2020, 33, pp.101162. ⟨10.1016/j.addma.2020.101162⟩. ⟨hal-02540295⟩

Nicolas Milon, Juan-Luis Fuentes Rojas, Adrien Castinel, Laurent Bigot, Géraud Bouwmans, et al.. A tunable filter for high molecular weight DNA selection and linked-read sequencing. Lab on a Chip, 2020, 20 (1), pp.175-184. ⟨10.1039/C9LC00965E⟩. ⟨hal-02400601⟩

Communications dans un congrès

Jean Cacheux, Aurélien Bancaud, Jose Ordonez-Miranda, Laurent Jalabert, Yukiko T Matsunaga. Collagen matrix remodeling by fibroblasts monitored by interstitial flow metrology. Microphysiological systems: from organoids to organ-on-chip, Apr 2020, Cargèse, France. ⟨hal-03821244⟩

Pré-publications, documents de travail

Olivier Liot, Catherine Sempere, Christophe Ybert, Anne-Laure Biance. Coupled electrokinetic transport through a nanoporous membrane: a theoretical approach. 2020. ⟨hal-02900453⟩

2019

Articles dans une revue

Antoine Naillon, Pierre Joseph, Marc Prat. Direct observation of pore collapse and tensile stress generation on pore walls due to salt crystallization in a PDMS channel. Soft Matter, 2019, 15, pp.4562-4569. ⟨10.1039/C8SM02546K⟩. ⟨hal-02363335⟩

Marius Socol, Renjie Wang, Daniel Jost, Pascal Carrivain, Cédric Vaillant, et al.. Rouse model with transient intramolecular contacts on a timescale of seconds recapitulates folding and fluctuation of yeast chromosomes. Nucleic Acids Research, 2019, 47 (12), pp.6195-6207. ⟨10.1093/nar/gkz374⟩. ⟨hal-02276944⟩

Rémi Malbec, Bayan Chami, Lorène Aeschbach, Gustavo Ruiz Buendía, Marius Socol, et al.. µLAS: Sizing of expanded trinucleotide repeats with femtomolar sensitivity in less than 5 minutes. Scientific Reports, 2019, 9, pp.23. ⟨10.1038/s41598-018-36632-5⟩. ⟨hal-01997319⟩

Marius Socol, Renjie Wang, Daniel Jost, Pascal Carrivain, Cédric Vaillant, et al.. Rouse model with transient intramolecular contacts on a timescale of seconds recapitulates folding and fluctuation of yeast chromosomes. Nucleic Acids Research, 2019, 47 (12), pp.6195-6207. ⟨10.1093/nar/gkz374⟩. ⟨hal-04550349⟩

Anaïs Chalard, Pierre Joseph, Sandrine Assié-Souleille, Barbara Lonetti, Nathalie Saffon-Merceron, et al.. Wet spinning and radial self-assembly of a carbohydrate low molecular weight gelator into well organized hydrogel filaments. Nanoscale, 2019, ⟨10.1039/C9NR02727K⟩. ⟨hal-02166311⟩

Loic Recoules, Sulivan Jouanneau, Gerald Thouand, Anne Marie Gué, Ali Boukabache. Towards a Miniaturized Device to Evaluate the BOD Parameter of Wastwater. International Journal of Environmental Science and Development, 2019, 10 (6), pp.178-182. ⟨10.18178/ijesd.2019.10.6.1168⟩. ⟨hal-02353122⟩

Nicolas Milon, Céline Chantry-Darmon, Carine Satge, Margaux-Alison Fustier, Stéphane Cauet, et al.. μLAS technology for DNA isolation coupled to Cas9-assisted targeting for sequencing and assembly of a 30 kb region in plant genome. Nucleic Acids Research, 2019, 47 (15), pp.8050-8060. ⟨10.1093/nar/gkz632⟩. ⟨hal-02338524⟩

Jean Cacheux, Aurélien Bancaud, Thierry Leichle, Pierre Cordelier. Technological Challenges and Future Issues for the Detection of Circulating MicroRNAs in Patients With Cancer. Frontiers in Chemistry, 2019, 7, ⟨10.3389/fchem.2019.00815⟩. ⟨hal-02392369⟩

Marius Socol, Hubert Ranchon, Bayan Chami, Antony Lesage, Jean-Marc Victor, et al.. Contraction and Tumbling Dynamics of DNA in Shear Flows under Confinement Induced by Transverse Viscoelastic Forces. Macromolecules, 2019, 52 (4), pp.1843-1852. ⟨10.1021/acs.macromol.8b02184⟩. ⟨hal-02076283⟩

Communications dans un congrès

Lyne Pillemont, Daniel Guneysu, Céline Elie-Caille, Wilfrid Boireau, Anne Marie Gué. Towards onchip EVs separation: a lab‐on‐chip approach. J Extracell Vesicles. International Society for Extracellular Vesicles, Apr 2019, Kyoto, Japan. ⟨hal-02991632⟩

Pierre Joseph, Marianne Elias, Fabien Mesnilgrente, Adrian Laborde, Debora Berti, et al.. Microfluidics to fabricate and probe vesicles mimicking cells. BioPhee - Membrane Biophysics of Exo-Endocytosis: From Model Systems to Cells, Apr 2019, Mandelieu la Napoule, France. ⟨hal-02166276⟩

Lyne Pillemont, Daniel Guneysu, Céline Elie-Caille, Wilfrid Boireau, Anne Marie Gué. Towards on-chip EVs separation: a lab-on-chip approach. Annual meeting of the International Society for Extracellular Vesicles (ISEV2019), International Society for Extracellular Vesicles, Apr 2019, Kyoto, Japan. pp.PS04.10. ⟨hal-02421493⟩

Marion Valette, Mathias Bouguelmouna, Mélanie Mariotte, Rémi Courson, Marie-Charline Blatché, et al.. ON-CHIP PRE-TREATMENT OF BIOLOGICAL SAMPLES FOR THE ISOLATION OF ADIPOSE STEM CELLS (ASCs). Transducers, Jun 2019, Berlin, Germany. ⟨10.1109/TRANSDUCERS.2019.8808694⟩. ⟨hal-02190131⟩

Autres documents

Marion Valette, Mathias Bouguelmouna, Mélanie Mariotte, Rémi Courson, Marie-Charline Blatché, et al.. Adipose Stem Cells (ASCs) isolation by on-chip pre-treatment of biological samples. Lab-On-Chip and Microfluidics Europe, Jun 2019, Rotterdam, Netherlands. 2019. ⟨hal-02190393⟩

Nous collaborons avec plusieurs groupes travaillant en oncologie et en biophysique. Nous portons le projet ERC UnderPressure, et participons à plusieurs projets européens et nationaux de l'ANR. Voir les pages sur les projets de recherche pour plus de détails.

Nous sommes également fortement impliqués dans le Réseau National de Microfluidique GDR Micro Nano Fluidics MNF.

MILE Team

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