Microsystems to measure water quality

The quantity of organic pollutants in water is evaluated by measuring the dissolved oxygen in water during 5 days (BOD5, biological Oxygen Demand) in presence of bacteria.  We are developing a new approach by associating microsystem technologies and biological techniques to obtain a more precise value of BOD in a shorter time. The bioMEMS chip developed has been designed as a multi-wells microfluidic device, and fabricated by combining PDMS and glass materials. To quantify the organic pollutants, we use specific bacteria and evaluate their oxygen consumption in order to measure/identify the pollutants concentrations. The monitoring of the bacterial activity is ensured by optical sensors or a fluorescent molecule (resazurin).

bioMEMS to read BOD   Optical reading of bacteria in 8 wells

References

1. L.Recoules, these de Doctorat soutenue le 22-09-2015 ;

2.Multi-bacteria based biomems to measure the BOP parameter of polluted waters, L.RECOULES, V.PICOT, S.JOUANNEAU, G.ANDRIEUX, G.THOUAND, Y.PRIMAULT, D.ESTEVE, A.BOUKABACHE,  Micromechanics and Microsystems Europe workshop ( MME ) 2014 du 31 août au 03 septembre 2014, Istanbul (Turquie), Août 2014, 5p. , N° 14505

3. Development of a bacteria based microsystem for a quickly measure of polluants in rejected water, L.RECOULES, V.PICOT, S.JOUANNEAU, S.ASSIE-SOULEILLE, D.LAGRANGE, G.THOUAND, Y.PRIMAULT, D.ESTEVE, A.BOUKABACHE, Microfluidic engineering of smart materials. Annual meeting of the GDR MicronanoSystèmes & MicroNanoFluidique 2013 du 16 décembre au 17 décembre 2013, Bordeaux (France), Décembre 2013