69documents trouvés
Electrokinetic mixers based on stimuli-responding surfaces
G.PAUMIER, J.SUDOR, E.COLLE, B.MARTY, A.BANCAUD, T.CAMPS, A.M.GUE
MIS
Manifestation avec acte : 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Paris (France), 7-11 Octobre 2007, pp.910-912 , N° 07455
Diffusable
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Mots-Clés / Keywords
Poly(N-isopropyl acrylamide); Thermoresponsive; Mixing; Microheaters;
Nucleosome chiral transition under positive torsional stress in single chromatin fibers
A.BANCAUD, G.WAGNER, N.CONDE E SILVA, C.LAVELLE, H.WONG, J.MOZZICONACCI, A.SIVOLOB, E.LE CAM, J.M.VICTOR, L.MOUAWAD, J.L.VIOVY, A.PRUNELL
MIS, Institut Curie, Institut Monod, LPTMC, Taras Shevchenko, LM2C, INSERM, Orsay
Revue Scientifique : Molecular Cell, Vol.27, N°1, pp.135-147, Juillet 2007 , N° 07572
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Abstract
Using magnetic tweezers to investigate the mechanical response of single chromatin fibers, we show that fibers submitted to large positive torsion transiently trap positive turns at a rate of one turn per nucleosome. A comparison with the response of fibers of tetrasomes (the [H3-H4]2 tetramer bound with 50 bp of DNA) obtained by depletion of H2A-H2B dimers suggests that the trapping reflects a nucleosome chiral transition to a metastable form built on the previously documented right-handed tetrasome. In view of its low energy, <8 kT, we propose that this transition is physiologically relevant and serves to break the docking of the dimers on the tetramer that in the absence of other factors exerts a strong block against elongation of transcription by the main RNA polymerase.
Mots-Clés / Keywords
DNA;
Chromatin structure and macromolecular confinement
A.BANCAUD, N.DAIGLE, J.MOZZICONACCI, J.ELLENBERG
MIS, EMBL
Manifestation avec acte : 51st Biophysical Society Meeting (2007), Baltimore (USA), 3-7 Mars 2007, 1p. , N° 07646
Diffusable
112006Implications of molecular crowding on nuclear protein dynamics
A.BANCAUD, N.DAIGLE, S.HUET, J.ELLENBERG
MIS, EMBL
Manifestation sans acte : Biological Diffusion and Brownian Dynamics Brainstorm, Heidelberg (Allemagne), 26-29 Mars 2007, 1p. , N° 07764
Diffusable
112632Molecular crowding slows down diffusion and enhances protein association to heterochromatin
A.BANCAUD, N.DAIGLE, S.HUET, J.MOZZICONACCI, J.BEAUDOUIN
MIS, EMBL, LPTMC, iBiOs-DKFZ
Manifestation sans acte : Japanese-French frontiers of Science, Kanagawa (Japon), 26-30 Janvier 2007, 1p. , N° 07573
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111761Implications of molecular crowding on nuclear protein-dynamics
A.BANCAUD, N.DAIGLE, S.HUET, J.ELLENBERG
MIS, EMBL
Manifestation sans acte : Biolateral Triangle Meeting, Paris (France), 19-19 Janvier 2007, 1p. , N° 07729
Diffusable
112532Automatic quantification of microtubule dynamics in living cells
L.SIRONI, J.SOLON, A.BANCAUD, D.BRUNNER, J.ELLENBERG
EMBL, MIS
Manifestation sans acte : American Society of Cell Biology, San Diego (USA), 9-13 Décembre 2006, 1p. , N° 06255
Diffusable
112045Torsion positive et fibre de chromatine: état chiral droit du nucléosome
A.BANCAUD, G.WAGNER, N.CONDE E SILVA, H.WONG, M.BARBI, J.MOZZICONACCI, C.LAVELLE, L.MOUAWAD, J.M.VICTOR, A.PRUNELL, J.L.VIOVY
Institut Curie, MIS, Institut Monod, LPTMC, INSERM, Orsay
Manifestation sans acte : 20ème Congrés de la Société Française de Biophysique, Anglet (France), 14-19 Octobre 2006, 2p. , N° 06254
Diffusable
112043Automatic quantification of microtubules dynamics by fluorescence microscopy in live cells
L.SIRONI, A.BANCAUD, J.SOLON, D.BRUNNER, J.ELLENBERG
EMBL, MIS
Manifestation sans acte : European Light Microscopy and Imaging Meeting, Ofir (Portugal), 30 Mai - 2 juin 2006, 1p. , N° 06256
Diffusable
112047