69documents trouvés
Structure de la chromatine et stabilité de l'hétérochromatine: une étude de diffusion en milieu fractal
A.BANCAUD, S.HUET, N.DAIGLE, J.MOZZICONACCI, J.BEAUDOUIN, J.ELLENBERG
N2IS, EMBL, iBiOs-DKFZ
Manifestation sans acte : Architecture Fonctionnelle du Chromosome Eukaryote, Paris (France), 9 Avril 2009, 1p. (Résumé) , N° 09317
Diffusable
117934Systèmes nanofluidiques. Technologies et applications
P.ABGRALL, A.BANCAUD, P.JOSEPH
N2IS
Revue Scientifique : Techniques de l'Ingénieur, N°NM250, pp.1-15, 2009, Dossiers Nanosciences et Nanotechnologies , N° 09172
Diffusable
122279Micro et Nanofluidique: une technologie transversale à fort potentiel applicatif
A.M.GUE, A.BOUKABACHE, P.JOSEPH, A.BANCAUD
N2IS
Ouvrage (contribution) : La Micro-Nanoélectronique. Enjeux et mutation, N°ISBN 978-2-271-06829-3, Janvier 2009, pp.243-248 , N° 09038
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116490Nanofluidics: technological developments and capillary invasion
Y.VIERO, A.ALLOUCH, M.GODDEFROY, J.C.MILLET, P.DUBREUIL, A.M.GUE, A.BANCAUD, P.JOSEPH, P.ABGRALL, N-T. NGUYEN
N2IS, TEAM, NTU, Nanyang
Manifestation sans acte : GDR Micro Nano Systèmes, Montpellier (France), 3 Décembre 2008, 1p. , N° 08835
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117918Nanofluidic systems: development of fabrication technologies and characterization of capillary filling rates
A.ALLOUCH, Y.VIERO, M.GODDEFROY, J.C.MILLET, P.DUBREUIL, A.BANCAUD, P.JOSEPH, A.M.GUE, P.ABGRALL, N-T.NGUYEN
N2IS, TEAM, Singapore
Manifestation avec acte : 1st European Conference on Microfluidics (MicroFlu'08), Bologne (Italie), 10-12 Décembre 2008, 8p. , N° 08700
Diffusable
Plus d'informations
Mots-Clés / Keywords
Nanofluidics; Technology; Capillary filling; Phase shift lithography; Micro and nano-bubble;
3D dynamic fluorescence microscopy based on fabricated V-shaped mirrors
H.HAJJOUL, J.GIRARD, M.DILHAN, I.LASSADI, K.BYSTRICKY, A.BANCAUD
TEAM, LBME, N2IS
Manifestation sans acte : Journées Nationales sur les Technologies Emergentes (JNTE), Toulouse (France), Novembre 2008, 2p. , N° 08836
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117920Novel approach for 3D live cell fluorescence microscopy based on microfabricated mirrors
H.HAJJOUL, J.GIRARD, M.DILHAN, S.KOCANOVA, K.BYSTRICKY, A.BANCAUD
N2IS, TEAM, LBME, MIS
Manifestation avec acte : 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS'08), San Diego (USA), 12-16 Octobre 2008, 2p. , N° 08181
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115617Nanofluidics: technological developments and capillary invasion
Y.VIERO, A.ALLOUCH, M.GODDEFROY, J.C.MILLET, P.DUBREUIL, A.M.GUE, A.BANCAUD, P.JOSEPH, P.ABGRALL, N-T. NGUYEN
N2IS, TEAM, NTU, Nanyang
Manifestation sans acte : 3èmes Journées Franco-espagnoles. IBERNAM-CMC, Toulouse (France), 16-17 Octobre 2008, 1p. , N° 08835
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117917Integration of an elastomer in micromachining technology
S.SOULIMANE, S.CHARLOT, D.BOURRIER, J.SUDOR, A.BANCAUD, A.M.GUE, H.CAMON
N2IS, TEAM, N2IS
Manifestation avec acte : Smart System Integration, Barcelone (Espagne), 9-10 Avril 2008, pp.533-536 , N° 08236
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113801Thermoresponsive polymer-based microdevice for nano-liquid chromatography
G.PAUMIER, S.SIV, A.BANCAUD, J.SUDOR, A.M.GUE
MIS
Manifestation avec acte : International Conference on Biomedical Electronics and Devices, Funchal (Portugal), 28-31 Janvier 2008, pp.178-181 , N° 07454
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Plus d'informations
Abstract
We report here on the development of an integrated device for sample desalting and pre-concentration for nanoLC / ESI-MS analysis combining poly-(N-isopropyl acrylamide) (PNIPAM) grafted microbeads and the means to dynamically control their temperature. Thermoresponsive properties of PNIPAM induce switchable hydrophobic/hydrophilic surfaces on which peptides can reversibly adsorb and desorb. The device is fabricated on a glass or pyrex substrate with deposited Ti/Au electrodes serving as built-in resistive heating sources. Premolded microfluidic channels and reservoirs made in PDMS are eventually assembled. Electrical and thermal characterization together with multiphysics modeling have been performed. The SiO2 surfaces of the channels and silica beads used as carriers of the stationary phases have been end-grafted with PNIPAM and employed to study the reversible adsorption and release kinetics of albumin-fluorescein conjugates by fluorescence video microscopy. It is clearly shown albumin-fluorescein complexes adsorb on beads surfaces above the transition temperature of PNIPAM (hydrophobic state), and are released when the temperature decreases (hydrophilic state), yet not fully reversibly.
Mots-Clés / Keywords
PNIPAM; Thermoresponsive polymer; NanoLC;