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Abstract

The methods presented can be used for non-photostructurable polymers and where spin-coating cannot be performed. As demonstrator of the viability of the proposed fabrication process, they have been applied for the definition of hydrophobic barriers on a microfluidics network, which is dedicated to selectively dispense liquid to a spotting device consisting of 12 silicon microcantilevers.

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Abstract

For the purpose of biochip miniaturization, we developed a liquid spotter-based on silicon microcantilevers incorporating in-plane nanotips as a MEMS nanopatterning tool. We present new fabricated cantilevers with added piezoresistors for the contact force control during deposition. Matrices of droplets of 1 ¼m in diameter were achieved with a 10 ¼m interspot distance with high uniformity and high reproducibility by the nanotip cantilever with piezoresistive force controller.

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Abstract

This work reports on a complementary use of surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D) technologies to study interactions between a peptide antigen and polyclonal antibodies, in an experimental format suitable for diagnostic assays of autoimmune diseases. In the chosen model, a synthetic peptide from the juxtamembrane region of IA-2 (a type 1 diabetes associated antigen) was immobilized by an optimized chemical protocol applicable to both BIACORE and QCM-D sensors. A thorough study of the peptide immobilization was performed to optimize the signal-to-noise ratio using mixed self-assembled monolayers (SAM) on a gold surface. Introduction of polyethylene glycol (EG6) chains into mixed SAM layers and addition of an anionic surfactant to the human serum reduced non-specific binding without modifying the viscoelasticity properties of the layer. Under our conditions, the antibody SPR detection limit was determined to be 0.2 nM in diluted human serum. This value is in agreement with the reported rank distribution of IA-2 antibodies in diabetic patient sera. Label-free and real-time technologies such as SPR and/or QCM-D could be precious tools in future diagnostic assays.

Abstract

Although extensively presented as one of the most promising silicon-based micromachined sensor adapted to real-time measurements in liquid media, the cantilevered structure still suffers from its quality factor (Q) dramatic dependence on the liquid viscosity thus lowering the measurement resolution. In this paper, micromachined piezoelectric membranes are introduced as a potential alternative to the cantilevers for biological applications. High Q-factors (up to 150) of micromachined piezoelectric membranes resonating in various liquid mixtures (water/glycerol and water/ethanol) are thus reported and a theoretical model proposed by Lamb [H. Lamb, On the vibrations of an elastic plate in contact with water, Proc. Roy. Soc. Lond. A 98 (1920) 205216] is validated for microscale structures proving that the variation of the liquid viscosity (if lower than 10 cP) has no effect on the dynamic behavior of the membranes. To conclude, two types of experiments were performed in water/glycerol mixtures: in-flow (with liquid continuously flowing on the devices) and in-spot (with individual membranes oscillating in a 5 ¼L volume of liquid). The results interestingly showed that for the in-spot configuration the Q-factor values are more than two-fold the ones corresponding to in-flow measurements thus providing alternative insights into the way to conceive ideal configurations for real-time biological measurements in liquid media.

Abstract

We have used fountain pen microlithography to deposit arrays of molecularly imprinted polymer microdots on flat substrates. We visualize analyte binding to the dots by fluorescence microscopy with the aid of fluorescein as a model analyte. Elution and readsorption of the analyte to the MIP dots were possible if the porosity of the dots was improved by a sacrificial polymeric porogen. The imprinting effect was confirmed by using compounds structurally related to fluorescein. In addition, we show with another MIP specific to 2,4-D that, apart from the direct measurement of the binding of fluorescent compounds, a competitive immunoassay-type format can also be used to transduce the binding. We believe that this technique has a strong potential for the fabrication of biomimetic microchips and other types of integrated biosensors.