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Publications de l'équipe MH2F

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18445
28/09/2018

Solute Concentration Measurements in Diluted Solutions by means of Split Ring Resonators

P.VELEZ, J.MATA-CONTRERAS, D.DUBUC, K.GRENIER, F.MARTIN

UAB, MH2F

Manifestation avec acte : European Microwave Conference ( EuMC ) 2018 du 23 septembre au 28 septembre 2018, Madrid (Espagne), Septembre 2018, 4p. , N° 18445

Lien : https://hal.laas.fr/hal-01951438

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Abstract

This paper presents a sensing device, useful for the measurement of small concentrations of solute content in liquids, based on a splitter/combiner microstrip structure loaded with a pair of split ring resonators (SRRs) in a symmetric configuration. The operation principle is similar to differential sensing, where one of the SRRs is put in contact with the reference liquid (the solvent), whereas the solution (or liquid under test-LUT) is forced to flow on top of the other SRR, the sensing element. To this end, fluidic channels are added on top of the SRRs, which are covered by a dry film in order to avoid substrate absorption. The asymmetry, caused by the presence of solute content in the LUT, modifies the response of the structure with regard to the one with identical liquids (pure solvent) in both channels, and the solute concentration can be inferred. In this work, it is demonstrated that glucose and sodium concentrations as small as 1 g/L and 0.5 g/L, respectively, in DI water can be measured.

145879
18448
01/08/2018

Highly-Sensitive Microwave Sensors Based on Open Complementary Split Ring Resonators (OCSRRs) for Dielectric Characterization and Solute Concentration Measurement in Liquids

P.VELEZ, K.GRENIER, J.MATA-CONTRERAS, D.DUBUC, F.MARTIN

UAB, MH2F

Revue Scientifique : IEEE Access, Vol.6, pp.48321-48338, Août 2018 , N° 18448

Lien : https://hal.laas.fr/hal-01951402

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Abstract

Differential permittivity sensors based on a pair of uncoupled microstrip lines, each one loaded with an open complementary split ring resonator (OCSRR), are proposed in this paper. The sensing principle is based on the measurement of the cross-mode insertion loss, very sensitive to asymmetric loading. Thus, by loading one of the OCSRRs with the reference sample, and the other one with the sample under test (SUT), the difference in the complex permittivity between both samples generates an asymmetry that gives rise to mode conversion. From the measurement of the cross-mode transmission coefficient, the dielectric properties of the SUT can be determined, provided those of the reference sample are well known. It is shown that by adding fluidic channels on top of the OCSRRs, the proposed sensor is useful for the measurement of the complex dielectric constant of liquids, and experimental results in mixtures of ethanol and deionized (DI) water and methanol in DI water, as a function of the ethanol/methanol content, are provided. Due to the high sensitivity of the proposed differential sensor to detect small perturbations (asymmetries), the structure is also of interest for the accurate measurement of solute concentrations in liquid solutions. In this paper, the structure is applied to monitor sodium content in aqueous solutions, and it is found that sodium concentrations as small as 0.25 g/L can be resolved.

145885
18447
15/06/2018

Low Volume and Label-Free Molecules Characterization and Cell Monitoring with Microwave Dielectric Spectroscopy

K.GRENIER, A.TAMRA, A.ZEDEK, G.POIROUX, F.ARTIS, T.CHEN, W.CHEN, M.POUPOT, J.J.FOURNIE, D.DUBUC

MH2F, CRCT-INSERM

Manifestation avec acte : IEEE International Microwave Bio Conference ( IMBioC ) 2018 du 14 juin au 15 juin 2018, Philadelphie (USA), Juin 2018, 3p. , N° 18447

Lien : https://hal.laas.fr/hal-01951457

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Abstract

Microwave dielectric spectroscopy is an attractive technique for non-destructive characterization of materials. This paper focus on its development for molecular and cellular analysis using microscale sensors. It especially focuses on the possible selectivity of the technique through the characterization of bio-molecules in aqueous solution, as well as on the possible monitoring of cells reactions at the single cell level. This last capability opens the door to kinetic evaluation, which is particularly interesting and important to evaluate any stimulus on cells, such as drugs, electrical signals and even the presence of other cell.

145883
18446
15/06/2018

Label-free discrimination of human lymphoma cell sub-populations with microwave dielectric spectroscopy

K.GRENIER, F.ARTIS, M.POUPOT, J.J.FOURNIE, D.DUBUC

MH2F, CRCT-INSERM

Manifestation avec acte : IEEE International Microwave Symposium ( IMS ) 2018 du 10 juin au 15 juin 2018, Philadelphie (USA), Juin 2018, 4p. , N° 18446

Lien : https://hal.laas.fr/hal-01951446

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This paper deals with the label-free microwave die-lectric characterization of B lymphoma cell lines, the OCI-LY7, OCI-LY10 and OCI-LY18 ones, and demonstrates that the technique is able to discriminate these three sub-population cell lines with their different dielectric properties. Proof is given that such a differentiation is not due to a variation of cell size, viability or cells concentration, but to internal molecular differences. This constitutes a major result as it demonstrates for the first time that microwave dielectric spectroscopy is sufficiently sensitive using di-electric properties variations to distinguish very similar biological cell lines of the same phenotype, B lymphoma cell lines in our case, whereas such a detection is traditionally performed with marker-based and costly equipment or using heavy genetic methods.

145881
18268
01/04/2018

Lymphatic Vasculature Requires Estrogen Receptor- Signaling to Protect From Lymphedema

F.MORFOISSE, F.TATIN, B.CAHPUT, N.THERVILLE, C.VAYSSE, R.METIVIER, J.MALLOIZEL-DELAUNAY, F.PUJOL, A.C.GODET, F.DE TONI, F.BOUDOU, K.GRENIER, D.DUBUC, E.LACAZETTE, A.C.PRATS, J.GUILLERMET-GUIBERT, F.LENFANT, B.GARMY-SUSINI

I2MC, CHU Toulouse, CRCT-INSERM, IUCT-Oncopole, IGDR, UT1, MH2F

Revue Scientifique : Arteriosclerosis, Thrombosis, and Vascular Biology, Vol.38, N°6, pp.1346-1357, Avril 2018 , N° 18268

Lien : https://hal-univ-rennes1.archives-ouvertes.fr/hal-01863214

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Abstract

Objective Estrogens exert beneficial effect on the blood vascular system. However, their role on the lymphatic system has been poorly investigated. We studied the protective effect of the 17 estradiolthe most potent endogenous estrogenin lymphedemaa lymphatic dysfunction, which results in a massive fluid and fat accumulation in the limb. Approach and Results Screening of DNA motifs able to mobilize ERs (estrogen receptors) and quantitative real-time polymerase chain reaction analysis revealed that estradiol promotes transcriptional activation of lymphangiogenesis-related gene expression including VEGF (vascular endothelial growth factor)-D, VEGFR (VEGF receptor)-3, lyve-1, and HASs (hyaluronan synthases). Using an original model of secondary lymphedema, we observed a protective effect of estradiol on lymphedema by reducing dermal backflowa representative feature of the pathology. Blocking ER by tamoxifenthe selective estrogen modulatorled to a remodeling of the lymphatic network associated with a strong lymphatic leakage. Moreover, the protection of lymphedema by estradiol treatment was abrogated by the endothelial deletion of the receptor ER in Tie2-Cre; ERlox/lox mice, which exhibit dilated lymphatic vessels. This remodeling correlated with a decrease in lymphangiogenic gene expression. In vitro, blocking ER by tamoxifen in lymphatic endothelial cells decreased cell-cell junctions, inhibited migration and sprouting, and resulted in an inhibition of Erk but not of Akt phosphorylation. Conclusions Estradiol protection from developing lymphedema is mediated by an activation of its receptor ER and is antagonized by tamoxifen. These findings reveal a new facet of the estrogen influence in the management of the lymphatic system and provide more evidence that secondary lymphedema is worsened by hormone therapy.

144575
18452
21/03/2018

Dispositif de mesures hyperfréquences de cellules biologiques individuelles et suivi du comportement cellulaire vis à vis d'un stimulus thermique

A.ZEDEK, A.TAMRA, D.DUBUC, K.GRENIER

MH2F

Manifestation avec acte : Journées de Caractérisation Microondes et Matériaux ( JCMM ) 2018 du 19 mars au 21 mars 2018, Paris (France), Mars 2018, 3p. , N° 18452

Lien : https://hal.laas.fr/hal-01951735

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Résumé

Ce papier présente un dispositif, qui permet de caractériser diélectriquement dans la gamme des micro-ondes des cellules individualisées mesurées directement dans leur milieu de culture et de suivre le comportement des cellules suivant différents stimuli. Le biocapteur micro-ondes spécialement développé pour l'analyse de cellules uniques comporte une partie sensible constituée d'une coupure capacitive de 5 μm de large, sur laquelle la cellule est piégée à l'aide d'un bloqueur mécanique. Nous avons pu montrer que les valeurs extraites des contrastes capacitif et conductif de cellules uniques de 40 MHz à 40 GHz révèlent l'état pathologique de cellules. Une étude plus approfondie sur l'impact d'un stress thermique en fonction de la durée d'exposition a été menée et montre le possible suivi du comportement de cellules vis à vis d'un stimulus. Ce résultat complète les différents atouts présentés par la technique de spectroscopie diélectrique micro-onde, à savoir une analyse cellulaire non invasive et non destructrice.

145901
18007
01/02/2018

Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool?

H.MARTIN-YKEN, C.GIRONDE, S.DERICK, H.TAIANA DARIUS, C.FURGER, D.LAURENT, M.CHINAIN

LISBP, MH2F, ILM, Papeete, Toulouse III

Revue Scientifique : Environmental Research, Vol.162, pp.144-151, Février 2018 , N° 18007

Lien : https://hal.archives-ouvertes.fr/hal-01680541

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Abstract

Ciguatoxins (CTXs) are lipid-soluble polyether compounds produced by dinoflagellates from the genus Gambierdiscus spp. typically found in tropical and subtropical zones. This endemic area is however rapidly expanding due to environmental perturbations, and both toxic Gambierdiscus spp. and ciguatoxic fishes have been recently identified in the North Atlantic Ocean (Madeira and Canary islands) and Mediterranean Sea. Ciguatoxins bind to Voltage Gated Sodium Channels on the membranes of sensory neurons, causing Ciguatera Fish Poisoning (CFP) in humans, a disease characterized by a complex array of gastrointestinal, neurological, neuropsychological, and cardiovascular symptoms. Although CFP is the most frequently reported non bacterial food-borne poisoning worldwide, there is still no simple and quick way of detecting CTXs in contaminated samples. In the prospect to engineer rapid and easy-to-use CTXs live cells-based tests, we have studied the effects of CTXs on the yeast Saccharomyces cerevisiae, a unicellular model which displays a remarkable conservation of cellular signalling pathways with higher eukaryotes. Taking advantage of this high level of conservation, yeast strains have been genetically modified to encode specific transcriptional reporters responding to CTXs exposure. These yeast strains were further exposed to different concentrations of either purified CTX or micro-algal extracts containing CTXs. Our data establish that CTXs are not cytotoxic to yeast cells even at concentrations as high as 1μM, and cause an increase in the level of free intracellular calcium in yeast cells. Concomitantly, a dose-dependent activation of the calcineurin signalling pathway is observed, as assessed by measuring the activity of specific transcriptional reporters in the engineered yeast strains. These findings offer promising prospects regarding the potential development of a yeast cells-based test that could supplement or, in some instances, replace current methods for the routine detection of CTXs in seafood products

142177
15044
11/12/2017

Microwave biosensors for non-invasive molecular and cellular investigations

K.GRENIER, D.DUBUC

MH2F

Ouvrage (contribution) : Medical and Biological Microwave Sensors and Systems, Cambridge University Press, N°ISBN 9781107297302, Décembre 2017, 25p. , N° 15044

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Abstract

In this comprehensive work, experts in the field detail recent advances in medical and biological microwave sensors and systems, with chapters on topics such as implantable sensors, wearable microwave tags, and UWB technology. Each chapter explores the theory behind the technology, as well as its design and implementation. This is supported by practical examples and details of experimental results, along with discussion of system design, design trade-offs, and possible constraints and manufacturing issues. Applications described include intracranial pressure monitoring, vital signs monitoring, and non-invasive molecular and cellular investigations. Presenting new research and advances in the field, and focusing on the state of the art in medical and biological microwave sensors, this work is an invaluable resource for enthusiastic researchers and practicing engineers in the fields of electrical engineering, biomedical engineering, and medical physics.

141743
17358
15/10/2017

Microwave microfluidic sensor based on a microstrip splitter/combiner configuration and split ring resonators (SRR) for dielectric characterization of liquids

P.VELEZ, L.SU, K.GRENIER, J.MATA-CONTRERAS, D.DUBUC, F.MARTIN

MH2F, UAB

Revue Scientifique : IEEE Sensors Journal, Vol.17, N°20, pp.6589-6598, Octobre 2017 , N° 17358

Lien : https://hal.archives-ouvertes.fr/hal-01597713

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Abstract

A microwave microfluidic sensor for dielectric characterization of liquids in real time is presented in this paper. The sensor is implemented in microstrip technology and consists of a symmetric splitter/combiner configuration loaded with a pair of identical split ring resonators (SRRs) and microfluidic channels placed on top of them (gap region). The sensor works in differential mode and sensing is based on frequency splitting. Thus, if the structure is unloaded or if it is symmetrically loaded with regard to the axial plane, only one transmission zero (notch) in the frequency response appears. However, if the axial symmetry is disrupted (e.g., by the presence of different liquids in the channels), two transmission zeros arise, and the difference in magnitude (notch depth) and frequency between such transmission zeros is indicative of the difference in the dielectric properties (complex dielectric constant). A circuit schematic, including transmission line sections to describe the distributed components, lumped elements to account for the SRRs and their coupling to the lines and lumped elements to model the liquid properties, is presented and validated. After proper calibration, the functionality of the proposed sensor is demonstrated by measuring the complex permittivity in solutions of deionized (DI) water and ethanol as a function of the ethanol content. Index Terms— Microwave sensors, differential sensors, microfluidics, dielectric characterization of liquids, permittivity measurements, microstrip technology, split ring resonator (SRR).

141177
17361
22/09/2017

Modeling and analysis of pairs of open complementary split ring resonators (OCSRRs) for differential permittivity sensing

P.VELEZ, L.SU, J.MATA-CONTRERAS, F.MARTIN, K.GRENIER, D.DUBUC

MH2F, UAB

Manifestation avec acte : IEEE MTT-S International Microwave Workshop Series on Advanced Materials and Processes ( IMWS-AMP ) 2017 du 20 septembre au 22 septembre 2017, Pavie (Italie), Septembre 2017, 3p. , N° 17361

Lien : https://hal.laas.fr/hal-01597746

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This paper is focused on the modeling and analysis of a pair of open complementary split ring resonators (OCSRR) for sensing purposes. Since the capacitance of the OCSRR is very sensitive to dielectric loading, it follows that the OCSRR pair is very useful for differential permittivity measurements. The proposed sensing approach is based on the measurement of the cross-mode S-parameters, particularly the cross-mode insertion loss, very sensitive to asymmetric loading. On the basis of the circuit model of the OCSRR, analytical expressions for the cross-mode insertion loss under small perturbations (asymmetries) are derived. Such expressions, of interest for sensor design, are validated through circuit and electromagnetic simulations.

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